Insulin is the only hormone in the body that lowers blood sugar and promotes the synthesis of glycogen, fat and protein. Exogenous insulin is mainly used in the treatment of diabetes mellitus and plays an irreplaceable role in the history of diabetes mellitus.
1 clarification:
The main objective of the clarification process is to remove cellular and granular impurities in the cell harvest fluid. The impurities are removed by the traditional process of two-stage centrifugation. At present, deep filtration is widely used in clarifying process steps.
Deep-bed filtration is the use of porous media from the mobile phase interception of solid particles rather than the surface interception, and the general medium surface with a positive charge, can be through electrostatic adsorption to intercept impurity particles.
Applicable products: SaiClear P series and S series clarifier filter, understand details →
2 ultrafiltration:
The main purpose of ultrafiltration is to concentrate the sample and transfer the solution into the preparation buffer. In addition to the conventional flux, the shear resistance of the target protein should be concerned.
Applicable Products: Teomax RC D channel ultrafiltration membrane package, understand the details →
3 sterilization and filtration:
Filtration is a process that removes microorganisms from a liquid stream without adversely affecting the quality of the product. The bacteria in liquid or air can be removed by physical retention filter to achieve the goal of Asepsis. The class of the sterilizing filter has been controversial, mainly because of the lack of consistency among manufacturers in measuring aperture. The definition method for classifying a bactericidal grade filter according to pore size has
Its limitations, so the regulation is based on the bacterial retention capacity to define the level of filter. PDA TR26 states that, under specified conditions, interception is per square centimeter within the effective filter surface area
B. Diminuta ATCC 19146 capacity of 107 filters are defined as bactericidal grade filters.
Applicable products: sterilization and filtration of terminal feed: SaiPress 0.22 μm for details →
Medium filtration: SaiPress 0.45 + 0.22 μm for details →
Buffer filtration and pre-column protection: Polysai pre-filter, SaiPress 0.22 μm for details →
Gas Filter & Respirator: Filgiant 0.22 μm for details →